Review, evaluation, and discussion of the challenges of missing value imputation for mass spectrometry-based label-free global proteomics.

Citation

Webb-Robertson, B.-J. M.; Wiberg, H. K.; Matzke, M. M.; Brown, J. N.; Wang, J.; McDermott, J. E.; Smith, R. D.; Rodland, K. D.; Metz, T. O.; Pounds, J. G.; Waters, K. M.; et al. Review, evaluation, and discussion of the challenges of missing value imputation for mass spectrometry-based label-free global proteomics. J. Proteome Res. 2015, 14 (5), 1993−2001.

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Summary

Evaluation of performance and caveats of 9 imputation algorithms applied on a LC-MS data set.

Study outcomes

Outcome O1

Most imputation methods perform well, no single algorithm or imputation strategy (single, local, global) outperforms, sometimes even no imputation is superior in subsequent classification analysis.

Outcome O2

Local similarity-based approaches are in general the most accuarate and robust methods. Such as least-squares adaptive (LSA) or regularized expectation maximization (REM) (Figure 4)

Outcome O3

The 'best' imputation method highly depends on the data and the goal of the downstream analysis and therewith advantageous methods are hard to define (Figure 3)

Further outcomes

With left-censored data the number of missing values highly depends on peptide intensity (Figure 1)

Study design and evidence level

General aspects

3 single-value approaches (LOD1,LOD2,RTI), 5 local similarity approaches (KNN, LLS, LSA, REM, MBI) and 2 global-structure approaches (PPCA, BPCA) were evaluated which allows comparison and discussion of different imputation strategies. They were applied to 3 real datasets of different type and species, which represent a broad biological application.